Application

Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.

For detection of biotinylated compounds.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.

Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide